Despite disinfection measures, rooms with a history of colonized or infected patients are under doubt and risk being the source of infection for subsequent patients. In order to reduce Hospital Acquired Infections, a reduction in surface bioburden is highly essential. 

Quality standards for disinfection and microbiological surface screening are some of the best ways to improve surface hygiene and thus, help prevent such infections. For whose rescue comes these tests like iso 22196 jis z 2801 tests.

The Japanese Industrial Standard Committee (JIS) tests by a global organization create and standardize test methods for various products and materials. The technique is highly diverse and adaptable, and it can help test the antimicrobial activity of a wide range of materials, including plastic products, metals, and ceramic materials.

But why are these tests essential?

The iso 22196 jis z 2801 method is the most widely used in the US and has become one of the industry-standard tests for checking the antimicrobial hard surface performance. The technique employed in this JIS Z 2801 method assesses the ability of antimicrobial surfaces such as plastics, metals, ceramics, and other materials to inhibit or kill microorganisms. The procedure then undergoes antimicrobial activity. Here it has a variety of real-world applications, ranging from hospitals and clinics to consumer companies concerned about the ability of a material to support bacterial growth.

So, let’s begin and understand everything about the iso 22196 jis z 2801 test method, as well as some of its pros and cons.

JIS Z 2801 ISO 22196: What is it?

The Japanese Industrial Standard (JIS) Z 2801 is a quantitative antimicrobial test for plastic materials, foams, and fabrics that are also known to give the same effect as ISO 22196. The JIS Z 2801 standard method measures the growth after certain common bacteria are inoculated into hard surfaces and plastics.

Technically, JIS Z2801 is currently based on ISO 22196. And these tests are the same methods published by two different organizations: the Japanese Industrial Standard (JIS) and the International Organization for Standardization (ISO). 

And why is it so reliable?

The JIS Z 2801 test method evaluates the ability of hard surfaces to inhibit or kill microorganisms over 24 hours of contact. Thus, being one of the most durable and reliable methods of testing.

What is the specific purpose of JIS Z 2801/ISO 22196?

Simply put, ISO 22196 is a test method for measuring antibacterial activity on plastics and other nonporous surfaces quantitatively. Based on the Japanese Industrial Standard (JIS) Z 2801, it is a reasonably sensitive assay capable of detecting low levels of antibacterial activity with repeatable results.

What Is the working mechanism of JIS Z 2801/ISO 22196?

  • ‌This testing will be first carried out following Test method Z 2801 of the Japanese International Standards Association. To ensure a contact area of approximately 40mm x 40mm, sections of the test material will be disinfected with an ethanol solution, inoculated with a liquid bacterial suspension, and covered with a sterile film (or equivalent). In a humidified chamber, the inoculation sections incubate for a specific period.
  • ‌‌Following incubation, the bacteria from each section are kept into a neutralizing solution and dilution-plated on nutritive agar to provide measurable viable bacterial burden data for each section. 
  • Lastly comes the step to check the effectiveness of antibacterial agents determined by rinsing the surface with a liquid neutralization medium, where any remaining bacteria can be found furthermore. 
  • It compares the reduction in bacteria on treated surfaces to the control sample result or the initial amount applied to the sample.
  • All of these steps thus show the bacteriostatic, bactericidal, or bacterial growth-supporting activities.

Final Data Analysis :

Test validity confirmation is done using in-house control results. The quantifiable microbial burden left on each sample is generally calculated in colony-forming units per gram of material (CFU/g). The data examined for the replicate samples in each set are 6 (n=6) consistently, but more are found if needed. Data analysis helps determine the percent and log reductions in microbial burdens on each test material compared to the negative control in each test condition.

Major organisms utilized in JIS Z 2801/ISO 22196 

Staphylococcus aureus (ATCC 6538P), Klebsiella pneumonia ( ATCC 4352), and Escherichia coli (ATCC 8739) are some of the organisms that are commonly used in this method. 

However, depending on the client’s needs, the number of specimens can also differ, thus allowing for testing against more products and commercially relevant microorganisms like MRSA infective organism, Pseudomonas aeruginosa, Campylobacter spp, or Salmonella spp.

Antimicrobial Test JIS Z 2801/ISO 22196 Turnaround Time

Testing the commonly used organisms described in the JIS Z 2801/ISO 22196 test standards will take approximately three to four working days.


  • One of the first antimicrobial test methods ever devised. JIS Z 2801 is a Japanese standard for materials and products entering the market. Antimicrobial tests that meet JIS standards are reliable and widely accepted.
  • For a wide range of markets, JIS Standards are always in use.ISO 22196 is a harmonized version of JIS Z 2801 that is almost identical to JIS Z 2801.
  • The Triplicate experimentation method of this test helps researchers estimate the precision of individual tests and improves overall experimental accuracy.


  • Microbial contaminants, for the most part, dry quickly on surfaces. Thus, reducing the amount of time an aqueous medium is available to facilitate interaction between the antimicrobial surface and microorganisms. As a result, JIS Z 2801 is a “best-test-case” for many products.
  • Since “control” plastics often have low antimicrobial activity, finding an ideal control surface can be challenging at times.

Also read: Why is it essential to undergo an iso antimicrobial test?

Tanya Jain

Hello! I'm a freelance writer with over 7 years of experience in all domains. I have a passion for words and reading- it's what inspires me to create the best possible content.

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